The antibiotic sensitivity test was also suggestive for Klebsiella pneumoniae because of ampicillin resistance which is intrinsically resistant. Lysine decarboxylase test-Positive Antibiotic sensitivity test Citrate Utilization Testįermentation of sugars (glucose, lactose, and mannitol )- acid and gas Note: Klebsiella oxytoca is indole positive. Non-motile because of only strict growth around the stab line Nitrate reduction test- Reduced to nitrite Non-motile Biochemical tests of Klebsiella pneumoniae on MacConkey’s agar Short thick Gram-negative rods Motility by hanging drop preparation method The mucoid nature of colonies is due to capsular material produced by the organisms. On MacConkey agar, the colonies appeared large, mucoid, and pink in color. Klebsiella pneumoniae on MacConkey’s agar The positive bottle was then sub-cultured into solid media Chocolate agar, blood agar, and MacConkey agar.Īfter overnight incubation, growth was seen in all three plates. Klebsiella pneumoniae isolated from septicemic patient blood culture on the basis of the following tests:-īlood culture bottle incubated into Bactec.Īfter 23 hours of incubation, Bactec indicated positive. Isolation of Klebsiella pneumoniae in Laboratory It may cause severe bronchopneumonia, urinary tract infections, nosocomial infections, wound infections, septicemia, meningitis, and rarely diarrhea too. Klebsiella pneumoniae subspecies rhinoscleromatis.Klebsiella pneumoniae subspecies pneumoniae.Klebsiella pneumoniae subspecies ozaenae.Subspecies of Klebsiella pneumoniae are.Klebsiella pneumoniae scientific classification is as follows They are found everywhere in nature and also in bacterial flora in our intestines. Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacteria with a prominent polysaccharide-based capsule and having size o,f 0.5 -0.8 µm wide to 1 -2 µm. Klebsiella is from the surname of German-Swiss microbiologist Edwin Klebs (1834–1913).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |